Human Cathepsin H ELISA Kit PicoKine®

Mfr.No.

EK0674

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.140	0.211	0.283	0.373	0.611	1.025	1.622	2.382

Description

Human Cathepsin H ELISA Kit PicoKine® (96 Tests). Quantitate Human CTSH in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Pro-cathepsin H; Cathepsin H mini chain; Cathepsin H; Cathepsin H heavy chain; Cathepsin H light chain; CTSH; CPSB

Immunogen

Expression system for standard: CHO; Immunogen sequence: A23-V335

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Cathepsin H

Subcellular Localization

Lysosome.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	372	987	40395	395	1022	3714
Standard deviation	16.74	68.1	1622.919	22.91	80.73	185.7
CV (%)	4.5%	6.9%	4.1%	5.8%	7.9%	5%

Protein Function

Important for the overall degradation of proteins in lysosomes.

Background

Cathepsin H is a protein that in humans is encoded by the CTSH gene. The protein encoded by this gene gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alteate splicing of this gene results in multiple transcript variants encoding different isoforms.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	372	315	318	335	335	22.68	6.7%
Sample 2	987	987	1084	1153	1052	70.12	6.6%
Sample 3	4095	4519	4622	4221	4364	214.12	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1512
Gene Name	CTSH
UniProt ID	P09668

Applications	ELISA
Research Area	Alzheimer's Disease,Cell Adhesion Proteins,Membrane Proteins,Neurodegenerative Disease,Neurology Process,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001351	EK0674	96 wells/kit	$499		Add to Cart

Human Cathepsin H ELISA Kit PicoKine®

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