Human Cathepsin D - CATH-D ELISA Kit

Mfr.No.

E4668Hu

Sensitivity

5.53ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Cathepsin D (also known as Cath-D) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Cathepsin D;Cathepsin D heavy chain;Cathepsin D light chain;CTSD

Molecular Weight

44552

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4668Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	285.3	13.5	4.7
Intra-Assay	2	18	570.2	19	3.3
Intra-Assay	3	18	247.1	12.6	5.1

Background

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed: 27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Protocol

These standard curves of E4668Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.402	2.392	2.337
	2.383
600ng/L	1.584	1.563	1.508
	1.543
300ng/L	0.926	0.847	0.792
	0.769
150ng/L	0.443	0.42	0.365
	0.397
75ng/L	0.262	0.226	0.171
	0.191
0ng/L	0.057	0.055	0
	0.053

Gene ID	1509
UniProt ID	P07339

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105964	E4668Hu	48T	$320		Add to Cart
BHE12105964	E4668Hu	96T	$458		Add to Cart

Human Cathepsin D - CATH-D ELISA Kit

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