Human Catechol-O-methyltransferase

Mfr.No.

E3731Hu

Sensitivity

0.095ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Catechol-O-Methyltransferase (also known as COMT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Catechol O-methyltransferase;COMT

Molecular Weight

30037

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3731Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.8	0.79	5.3
Intra-Assay	2	18	18.9	0.71	3.8
Intra-Assay	3	18	8.3	0.47	5.7

Background

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Protocol

These standard curves of E3731Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.051	2.111	2.058
	2.17
20ng/ml	1.431	1.431	1.379
	1.432
10ng/ml	0.852	0.795	0.742
	0.738
5ng/ml	0.485	0.479	0.427
	0.473
2.5ng/ml	0.267	0.258	0.205
	0.248
0ng/ml	0.038	0.053	0
	0.067

Gene ID	1312
UniProt ID	P21964

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105192	E3731Hu	48T	$320		Add to Cart
BHE12105192	E3731Hu	96T	$458		Add to Cart

Human Catechol-O-methyltransferase - COMT ELISA Kit

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