Human Carbonyl Reductase Nadph 1

Mfr.No.

E6599Hu

Alternative Names

20-beta-hydroxysteroid dehydrogenase;Carbonyl reductase (NADPH) 1;CBR;CBR1;CRN;hCBR1;NADPH-dependent carbonyl reductase 1;PG-9-KR;Prostaglandin 9-ketoreductase;Prostaglandin-E(2 9-reductase;SDR21C1;Short chain dehydrogenase/reductase family 21C member 1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

30375

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.31ng/L

Background

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol (PubMed:18449627, PubMed:15799708, PubMed:17912391, PubMed:7005231). Can convert prostaglandin E to prostaglandin F2-alpha (By similarity). Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione (PubMed:18826943, PubMed:17344335).

Gene ID	873
UniProt ID	P16152
Antigen	CBR1

Research Area

Signal Transduction; Cell Biology; Metabolism

Protocol

These standard curves of E6599Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.919	1.857	1.8
	1.796
1200ng/L	0.961	1.023	0.966
	1.085
600ng/L	0.509	0.546	0.489
	0.583
300ng/L	0.285	0.225	0.168
	0.165
150ng/L	0.147	0.134	0.077
	0.121
0ng/L	0.056	0.057	0
	0.058

Precision

We measured random samples of E6599Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	782.7	38.43	4.91
Intra-Assay	2	18	1894.49	60.32	3.18
Intra-Assay	3	18	928.01	35.64	3.84

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115347	E6599Hu	48T	$320		Add to Cart
BHE12115347	E6599Hu	96T	$458		Add to Cart

Human Carbonyl Reductase Nadph 1 - CBR1 ELISA Kit

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