Human Carbonic Anhydrase 9

Mfr.No.

E2273Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Carbonic Anhydrase 9 (also known as CA9) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CA 9;CA9;CAIX;CA-IX;Carbonate dehydratase IX;Carbonic anhydrase 9;Carbonic anhydrase IX;Membrane antigen MN;P54/58N;pMW1;RCC-associated antigen G250;Renal cell carcinoma-associated antigen G250

Molecular Weight

49698

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2273Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.4	0.24	4.4
Intra-Assay	2	18	11.6	0.47	4
Intra-Assay	3	18	4.5	0.27	6.1

Background

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

Protocol

These standard curves of E2273Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.501	2.535	2.488
	2.568
10ng/ml	1.568	1.467	1.421
	1.366
5ng/ml	0.777	0.825	0.779
	0.873
2.5ng/ml	0.385	0.406	0.36
	0.428
1.25ng/ml	0.252	0.267	0.22
	0.282
0ng/ml	0.062	0.047	0
	0.032

Gene ID	768
UniProt ID	Q16790

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103813	E2273Hu	48T	$320		Add to Cart
BHE12103813	E2273Hu	96T	$458		Add to Cart

Human Carbonic Anhydrase 9 - CA9 ELISA Kit

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