Human Carbonic Anhydrase 4

Mfr.No.

E2270Hu

Sensitivity

0.093ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Carbonic Anhydrase IV (also known as CA4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CA 4;CA4;CA-IV;Carbonate dehydratase IV;Carbonic anhydrase 4;Carbonic anhydrase IV

Molecular Weight

35032

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2270Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12	0.54	4.5
Intra-Assay	2	18	24.5	0.73	3
Intra-Assay	3	18	18.5	0.89	4.8

Background

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.

Protocol

These standard curves of E2270Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.005	1.918	1.874
	1.831
16ng/ml	0.915	0.966	0.923
	1.018
8ng/ml	0.588	0.501	0.458
	0.414
4ng/ml	0.19	0.181	0.138
	0.173
2ng/ml	0.116	0.096	0.053
	0.077
0ng/ml	0.027	0.044	0
	0.061

Gene ID	762
UniProt ID	P22748

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103810	E2270Hu	48T	$320		Add to Cart
BHE12103810	E2270Hu	96T	$458		Add to Cart

Human Carbonic Anhydrase 4 - CA4 ELISA Kit

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