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Home / ELISA Kit

Human Carbamoyl-phosphate Synthase Ammonia - CPS1 ELISA Kit

SKU : BHE12115344

Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.

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Mfr.No.

E6595Hu

Sensitivity

15.96ng/mL

Detection Range

30-9000ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Carbamoyl-phosphate Synthase (ammonia), mitochondrial;Carbamoyl-phosphate synthetase I;CPS1;CPSase I;CPSASE1;GATD6;PHN

Molecular Weight

164939

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6595Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4709.26	202.45	4.3
Intra-Assay	2	18	2772.97	135.93	4.9
Intra-Assay	3	18	1538.35	71.55	4.65

Background

Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.

Protocol

These standard curves of E6595Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/mL	1.698	1.729	1.681
	1.76
2400ng/mL	1.144	1.011	0.963
	0.878
1200ng/mL	0.59	0.619	0.571
	0.647
600ng/mL	0.15	0.21	0.163
	0.27
300ng/mL	0.098	0.105	0.058
	0.112
0ng/mL	0.056	0.048	0
	0.039

Gene ID	1373
UniProt ID	P31327

Research Area	Tags & Cell Markers; Signal Transduction; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115344	E6595Hu	48T	$320		Add to Cart
BHE12115344	E6595Hu	96T	$458		Add to Cart

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