Human CANT1 ELISA Kit PicoKine®

Mfr.No.

EK2109

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.019	0.065	0.152	0.286	0.494	0.865	1.493	2.000

Description

Human CANT1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CANT1 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Soluble calcium-activated nucleotidase 1; SCAN-1; Apyrase homolog; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107; CANT1; SHAPY

Immunogen

Expression system for standard: NS0; Immunogen sequence: R63-I401

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CANT1

Subcellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein; Golgi stack membrane; Single-pass type II membrane protein; Cell membrane

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	301	1170	4798	307	1195	4812
Standard deviation	15.65	62.01	259.09	18.73	70.51	327.22
CV (%)	5.2%	5.3%	5.4%	6.1%	5.9%	6.8%

Protein Function

Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed:12234496, PubMed:15248776, PubMed:15006348, PubMed:16835225). Involved in proteoglycan synthesis (PubMed:22539336).

Background

CANT1?is a?gene?that encodes soluble calcium-activated?nucleotidase?1, an?enzyme, in humans. It is mapped to 17q25.3. This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alteatively spliced transcript variants have been noted for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	319	341	313	295	317	16.43	5.1%
Sample 2	1085	1189	1030	1018	1080	67.54	6.2%
Sample 3	5570	5244	5973	5762	5637	268.07	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	124583
Gene Name	CANT1
UniProt ID	Q8WVQ1
Tissue Specificity	Widely expressed.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001657	EK2109	96 wells/kit	$750		Add to Cart

Human CANT1 ELISA Kit PicoKine®

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