Human/Canine/Porcine Insulin ELISA Kit PicoKine®

Mfr.No.

EK2287

Sensitivity

<2 pmol/L

Detection Range

15.6 pmol/L - 1,000 pmol/L

Reactivity

Human, Canine, Porcine

Sample Volume

Concentration(pmol/L)	0	15.6	31.2	62.5	125	250	500	1,000
O.D.	0.046	0.084	0.197	0.281	0.461	0.888	1.251	2.012

Description

Human/Canine/Porcine Insulin ELISA Kit PicoKine® (96 Tests). Quantitate Human/canine/porcine INS in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 2 pmol/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F25-N110

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pmol/L)	28	93	474	25	118	274
Standard deviation	1.53	2.72	10.57	1.14	5.17	21.81
CV(%)	5.5%	2.9%	2.2%	4.5%	4.4%	8%

Background

Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the insulin (INS) gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

Reproducibility

Lots	Lot 1 (pmol/L)	Lot 2 (pmol/L)	Lot 3 (pmol/L)	Lot 4 (pmol/L)	Mean (pmol/L)	Standard Deviation	CV (%)
Sample 1	28	18	24	19	22	1.69	7.6%
Sample 2	93	93	112	85	96	6.96	7.3%
Sample 3	474	411	299	444	407	21.08	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3630
Antigen	Human/Canine/Porcine
Gene Name	INS
UniProt ID	P01308

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001836	EK2287	96 wells/kit	$499		Add to Cart

Human/Canine/Porcine Insulin ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human/Canine/Porcine Insulin ELISA Kit PicoKine®

Rate it now..!!