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Home / ELISA Kit

Human CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit

SKU : BHE13703366

The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans.
PRKAR2b is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity.

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Mfr.No.	KTE61150
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2B.
Alternative Names	PRKAR2B; PRKAR2; RII-BETA; H_RG363E19.2; WUGSC:H_RG363E19.2; cAMP-dependent protein kinase type-beta regulatory chain; cAMP-dependent protein kinase; regulatory subunit beta 2
Detection Method	Colorimetric
Kit Components	Human CAMP-dependent protein kinase type II-beta regulatory subunit microplate Human CAMP-dependent protein kinase type II-beta regulatory subunit standard Human CAMP-dependent protein kinase type II-beta regulatory subunit detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKAR2B. No significant cross-reactivity or interference between Human PRKAR2B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKAR2b is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity.
Notes	This Human CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2B in samples. An antibody specific for PRKAR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5577

Applications	ELISA
Applications Notes	This Human CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2B in samples. An antibody specific for PRKAR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703366	KTE61150	48 T	$339		Add to Cart
BHE13703366	KTE61150	96 T	$589		Add to Cart
BHE13703366	KTE61150	96 Tx5	$2629		Add to Cart
BHE13703366	KTE61150	96 Tx50	$24569		Add to Cart

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