Human Calponin 1 - CNN1 ELISA Kit

Mfr.No.

E2948Hu

Sensitivity

0.02ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Calponin-1 (also known as CNN1) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Basic calponin;Calponin H1, smooth muscle;Calponin-1;CNN 1;CNN1

Molecular Weight

33170

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2948Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.2	0.1	4.8
Intra-Assay	2	18	5.1	0.15	3
Intra-Assay	3	18	4.4	0.17	4

Background

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Protocol

These standard curves of E2948Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.029	2.066	2.012
	2.104
4ng/ml	1.147	1.113	1.058
	1.078
2ng/ml	0.404	0.466	0.411
	0.527
1ng/ml	0.293	0.305	0.251
	0.318
0.5ng/ml	0.122	0.104	0.049
	0.085
0ng/ml	0.057	0.055	0
	0.052

Gene ID	1264
UniProt ID	P51911

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104485	E2948Hu	48T	$320		Add to Cart
BHE12104485	E2948Hu	96T	$458		Add to Cart

Human Calponin 1 - CNN1 ELISA Kit

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