Human Calpain Small Subunit 1

Mfr.No.

E4115hu

Description

This sandwich kit is for the accurate quantitative detection of human Calpain Small Subunit 1 (also known as CAPNS1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Calcium-activated neutral proteinase small subunit;Calcium-dependent protease small subunit;Calcium-dependent protease small subunit 1;Calpain regulatory subunit;Calpain small subunit 1;CANP small subunit;CAPNS 1;CAPNS1;CDPS;CSS1

Reactivity

Human

Detection Range

0.05-15ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

28316

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.02ng/ml

Background

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Gene ID	826
UniProt ID	P04632
Antigen	CAPNS1

Research Area

Others

Protocol

These standard curves of E4115hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.127	2.104	2.048
	2.082
4ng/ml	1.294	1.406	1.349
	1.517
2ng/ml	0.738	0.736	0.68
	0.735
1ng/ml	0.426	0.413	0.356
	0.399
0.5ng/ml	0.251	0.235	0.178
	0.218
0ng/ml	0.061	0.056	0
	0.052

Precision

We measured random samples of E4115hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.2	0.25	4.1
Intra-Assay	2	18	1.9	0.1	5
Intra-Assay	3	18	2.5	0.16	6.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105520	E4115hu	48T	$320		Add to Cart
BHE12105520	E4115hu	96T	$458		Add to Cart

Human Calpain Small Subunit 1 - CAPNS1 ELISA Kit

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