Human Calcium-binding Mitochondrial Carrier Protein Aralar1

Mfr.No.

E6581Hu

Alternative Names

AGC1;ARALAR;Calcium-binding mitochondrial carrier protein Aralar1;DEE39;EIEE39;Mitochondrial aspartate glutamate carrier 1;SLC25A12;Solute carrier family 25 member 12

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

74762

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.62ng/L

Background

Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane (PubMed:11566871, PubMed:25410934). May have a function in the urea cycle (PubMed:11566871).

Gene ID	8604
UniProt ID	O75746
Antigen	SLC25A12

Research Area

Neuroscience; Tags & Cell Markers; Signal Transduction; Neuroscience; Metabolism

Protocol

These standard curves of E6581Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.264	2.266	2.225
	2.268
600ng/L	1.736	1.709	1.668
	1.683
300ng/L	0.986	1.04	0.999
	1.093
150ng/L	0.627	0.676	0.635
	0.725
75ng/L	0.439	0.457	0.416
	0.474
0ng/L	0.028	0.041	0
	0.054

Precision

We measured random samples of E6581Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	339.37	23.04	6.79
Intra-Assay	2	18	693.61	36.12	5.21
Intra-Assay	3	18	137.29	11.9	8.67

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115340	E6581Hu	48T	$320		Add to Cart
BHE12115340	E6581Hu	96T	$458		Add to Cart

Human Calcium-binding Mitochondrial Carrier Protein Aralar1 - SLC25A12 ELISA Kit

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