Matrices listed below were spiked with certain level of C3a and the recovery rates were calculated by comparing the measured value to the expected amount of C3a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-95
91
EDTA plasma(n=5)
88-101
96
heparin plasma(n=5)
91-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C3a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
C3a ELISA Kit allows for the in vitro quantitative determination of C3a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human C3a(Complement Component 3a) ELISA Kit