Human C1S ELISA Kit PicoKine®

Mfr.No.

EK1934

Sensitivity

<0.1 ng/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.025	0.173	0.347	0.676	1.261	1.603	2.171	1.928

Description

Human C1S ELISA Kit PicoKine® (96 Tests). Quantitate Human C1S in cell culture supeatants, serum, plasma (heparin, EDTA or citrate) , urine, human milk. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement C1s subcomponent; C1 esterase; Complement component 1 subcomponent s; C1S

Immunogen

Expression system for standard: NS0; Immunogen sequence: E16-D688

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human C1S

Subcellular Localization

Blood microparticle, Extracellular region

Sample Type

Cell culture supeatants, Serum, Plasma, Urine, Human milk

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1236	4483	22854	1393	4567	22796
Standard deviation	76.63	300.36	1028.43	82.19	324.26	980.23
CV (%)	6.2%	6.7%	4.5%	5.9%	7.1%	4.3%

Protein Function

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in tu, activate C2 and C4.

Background

Complement component 1s is a protein involved in the complement system. It is mapped to 12p13.31. This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1236	1246	1393	1346	1305	66.45	5.0%
Sample 2	4483	4300	3867	4110	4190	228.40	5.4%
Sample 3	22854	23761	20481	22796	22473	1212.07	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	716
Gene Name	C1S
UniProt ID	P09871

Applications	ELISA
Research Area	Complement,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001443	EK1934	96 wells/kit	$499		Add to Cart

Human C1S ELISA Kit PicoKine®

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