Human C-MET/HGFR ELISA Kit PicoKine®

Mfr.No.

EK0744

Sensitivity

<5 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.036	0.081	0.118	0.236	0.340	0.552	1.035	1.955

Description

Human C-MET/HGFR ELISA Kit PicoKine® (96 Tests). Quantitate Human MET in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hepatocyte growth factor receptor;HGF receptor;2.7.10.1;HGF/SF receptor;Proto-oncogene c-Met;Scatter factor receptor;SF receptor;Tyrosine-protein kinase Met;MET;

Molecular Weight

155541 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-R307 (alpha) & S308-T932 (beta)

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human C-MET

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	210	601	1336	204	644	1384
Standard deviation	8.61	31.25	84.16	10.81	39.28	95.49
CV (%)	4.1%	5.2%	6.1%	5.3%	6.1%	6.9%

Protein Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells.

Background

C-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	210	196	181	199	196	10.35	5.2%
Sample 2	601	676	644	633	638	26.8	4.2%
Sample 3	1336	1534	1527	1377	1443	88.23	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4233
Gene Name	MET
UniProt ID	P08581
Protein Name	Hepatocyte growth factor receptor
Tissue Specificity	Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. .
Sequence	Belongs to the protein kinase superfamily. Tyr protein kinase family.

Applications	ELISA
Research Area	Cancer,Cancer Susceptibility,Epigenetics and Nuclear Signaling,Oncoproteins,Oncoproteins/Suppressors,Protein Phosphorylation,Proto-Oncogenes,Receptor Tyrosine Kinases,Signal Transduction,Transcription,Tyrosine Kinases

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000600	EK0744	96 wells/kit	$499		Add to Cart

Human C-MET/HGFR ELISA Kit PicoKine®

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