Human C-C Motif Chemokine 2

Mfr.No.

E4830Hu

Alternative Names

C-C motif chemokine 2;CCL 2;CCL2;HC11;MCAF;MCP-1;Monocyte chemoattractant protein 1;Monocyte chemotactic and activating factor;Monocyte chemotactic protein 1;Monocyte secretory protein JE;Small-inducible cytokine A2

Reactivity

Human

Detection Range

5-1800ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

11025

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.09ng/L

Background

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed: 9837883, PubMed: 10587439, PubMed: 10529171). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed: 9837883, PubMed: 10587439). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed: 8627182, PubMed: 9792674, PubMed: 8195247). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed: 8107690).

Gene ID	6347
UniProt ID	P13500
Antigen	CCL2

Research Area

Immunology

Protocol

These standard curves of E4830Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.228	2.291	2.237
	2.354
480ng/L	1.427	1.456	1.402
	1.484
240ng/L	0.824	0.847	0.793
	0.87
120ng/L	0.5	0.452	0.398
	0.403
60ng/L	0.303	0.309	0.255
	0.315
0ng/L	0.05	0.054	0
	0.058

Precision

We measured random samples of E4830Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	616.6	22.37	3.6
Intra-Assay	2	18	205.2	10.83	5.3
Intra-Assay	3	18	268.7	13.16	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105685	E4830Hu	48T	$320		Add to Cart
BHE12105685	E4830Hu	96T	$458		Add to Cart

Human C-C Motif Chemokine 2 - CCL2 ELISA Kit

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