Matrices listed below were spiked with certain level of BTC and the recovery rates were calculated by comparing the measured value to the expected amount of BTC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
99
EDTA plasma(n=5)
92-102
98
heparin plasma(n=5)
86-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BTC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
BTC ELISA Kit allows for the in vitro quantitative determination of BTC concentrations in serum, plasma, tissue homogenates and other biological fluids.