Human Bromodomain-containing Protein 3

Mfr.No.

E6568Hu

Sensitivity

5.44ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BRD3;Bromodomain-containing protein 3;ORFX;RING3L;RING3-like protein

Molecular Weight

79542

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6568Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1054.79	54.46	5.16
Intra-Assay	2	18	569.29	27.12	4.76
Intra-Assay	3	18	254.59	10.95	4.3

Background

Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:18406326, PubMed:27105114). Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).

Protocol

These standard curves of E6568Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.674	1.784	1.727
	1.893
600ng/L	1.074	1.093	1.037
	1.112
300ng/L	0.467	0.549	0.492
	0.63
150ng/L	0.211	0.189	0.132
	0.166
75ng/L	0.072	0.079	0.023
	0.087
0ng/L	0.055	0.057	0
	0.058

Gene ID	8019
UniProt ID	Q15059

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115368	E6568Hu	48T	$320		Add to Cart
BHE12115368	E6568Hu	96T	$458		Add to Cart

Human Bromodomain-containing Protein 3 - BRD3 ELISA Kit

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