Human Branched-chain-amino-acid Aminotransferase - Mitochondrial

Mfr.No.

E4543Hu

Sensitivity

8.01ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BCAT 2;BCAT(m;BCAT2;Branched-chain-amino-acid aminotransferase, mitochondrial;Placental protein 18;PP18

Molecular Weight

44288

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4543Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	349.8	13.62	3.9
Intra-Assay	2	18	1030.8	34.92	3.4
Intra-Assay	3	18	685.5	30.17	4.4

Background

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

Protocol

These standard curves of E4543Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.028	1.954	1.917
	1.88
800ng/L	1.16	1.146	1.11
	1.133
400ng/L	0.49	0.533	0.496
	0.575
200ng/L	0.16	0.208	0.171
	0.256
100ng/L	0.106	0.119	0.083
	0.133
0ng/L	0.027	0.037	0
	0.047

Gene ID	587
UniProt ID	O15382

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105858	E4543Hu	48T	$320		Add to Cart
BHE12105858	E4543Hu	96T	$458		Add to Cart

Human Branched-chain-amino-acid Aminotransferase - Mitochondrial - BCAT2 ELISA Kit

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