Human BMP-7 ELISA Kit PicoKine®

Mfr.No.

EK0884

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.019	0.084	0.143	0.291	0.485	0.979	1.746	2.576

Description

Human BMP-7 ELISA Kit PicoKine® (96 Tests). Quantitate Human BMP7 in bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bone morphogenetic protein 7 (Osteogenic protein 1), isoform CRA_b ;cDNA FLJ78019, highly similar to Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1), mRNA ;cDNA, FLJ92758, Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1) (BMP7), mRNA ;BMP7 ;hCG_40100 ;

Molecular Weight

49313 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S293-H431

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human BMP-7

Sample Type

Bone tissue homogenate, Cell culture supernatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	56	263	982	53	244	937
Standard deviation	4.08	12.36	54.01	4.66	15.86	57.15
CV (%)	7.3%	4.7%	5.5%	8.8%	6.5%	6.1%

Background

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	56	49	57	56	54	3.2	5.9%
Sample 2	263	286	255	285	272	13.55	4.9%
Sample 3	982	921	916	948	941	26.23	2.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	655
Antigen	BMP-7
Gene Name	BMP7
UniProt ID	A8K571
Protein Name	Bone morphogenetic protein 7

Applications	ELISA
Research Area	Cancer,Cell Biology,Cell Cycle,Cell Differentiation,Cytoskeleton/ECM,Developmental Biology,Extracellular Matrix,Growth Factors/Hormones,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathways,Metabolism,Nervous System Development,Organogenesis,Pathways and Processes,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,Structures,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000677	EK0884	96 wells/kit	$499		Add to Cart

Human BMP-7 ELISA Kit PicoKine®

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