Human BMP-7 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0884

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.019	0.084	0.143	0.291	0.485	0.979	1.746	2.576

Description

Human BMP-7 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human BMP7 in bone tissue, cell culture supeatants, serum, plasma (heparin, EDTA) and urine.

Alternative Names

Bone morphogenetic protein 7; BMP-7; Osteogenic protein 1; OP-1; Eptotermin alfa; BMP7; OP1

Molecular Weight

49313 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S293-H431

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human BMP-7

Subcellular Localization

Secreted.

Sample Type

Bone tissue homogenate, Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	342	765	1108	372	780	1265
Standard deviation	19.8	42.8	57.6	30.5	62.4	98.7
CV (%)	5.8%	5.6%	5.2%	8.2%	8%	7.8%

Protein Function

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

Background

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	655
Gene Name	BMP7
UniProt ID	P18075
Protein Name	Bone morphogenetic protein 7
Tissue Specificity	Expressed in the kidney and bladder. Lower levels seen in the brain.

Applications	ELISA
Research Area	Extracellular Matrix,Growth Factors/Hormones,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathways,Metabolism,Nervous System Development,Organogenesis,Pathways and Processes,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,Structures,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000017	EZ0884	5 plates/kit	$500		Add to Cart

Human BMP-7 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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