Human BMP-4 ELISA Kit PicoKine®

Mfr.No.

EK0314

Sensitivity

<4 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.037	0.083	0.143	0.285	0.523	1.037	1.478	2.012

Description

Human BMP-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human BMP4 in cell culture supeatants, cell lysates and serum. Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bone morphogenetic protein 4;BMP-4;Bone morphogenetic protein 2B;BMP-2B;BMP4;BMP2B, DVR4;

Molecular Weight

46555 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S293-R408

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human BMP-4

Subcellular Localization

Secreted, extracellular space, extracellular matrix.

Sample Type

Cell culture supeatants, Cell lysates, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	196	814	1756	199	823	1888
Standard deviation	12.15	48.02	124.67	12.33	49.38	169.92
CV (%)	6.2%	7.1%%	7.1%	6.2%	6%	9%

Protein Function

Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity). .

Background

Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	196	183	176	194	187	8.16	4.3%
Sample 2	814	793	763	851	805	32.03	3.9%
Sample 3	1756	1763	1749	1644	1728	48.74	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	652
Gene Name	BMP4
UniProt ID	P12644
Protein Name	Bone morphogenetic protein 4
Tissue Specificity	Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
Sequence	Belongs to the TGF-beta family.

Applications	ELISA
Research Area	Cardiovascular,Developmental Biology,Growth Factors,Growth Factors/Hormones,Nervous System Development,Organogenesis,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000351	EK0314	96 wells/kit	$499		Add to Cart

Human BMP-4 ELISA Kit PicoKine®

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