Human Bleomycin Hydrolase - BLMH ELISA Kit

Mfr.No.

E4158hu

Description

This sandwich kit is for the accurate quantitative detection of human Bleomycin Hydrolase (also known as BLMH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BH;Bleomycin hydrolase;BLM hydrolase;BLMH;BMH

Reactivity

Human

Detection Range

5-1800ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

52562

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.62ng/L

Background

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.

Gene ID	642
UniProt ID	Q13867
Antigen	BLMH

Research Area

Cell Biology

Protocol

These standard curves of E4158hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.528	2.549	2.512
	2.57
480ng/L	1.434	1.422	1.385
	1.41
240ng/L	0.921	0.929	0.892
	0.938
120ng/L	0.466	0.459	0.422
	0.452
60ng/L	0.241	0.216	0.179
	0.191
0ng/L	0.045	0.037	0
	0.029

Precision

We measured random samples of E4158hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	120.2	8.37	7
Intra-Assay	2	18	351.9	17.94	5.1
Intra-Assay	3	18	406.1	14.32	3.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105559	E4158hu	48T	$320		Add to Cart
BHE12105559	E4158hu	96T	$458		Add to Cart

Human Bleomycin Hydrolase - BLMH ELISA Kit

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