Human Bikunin / alpha 1 Microglobulin ELISA Kit PicoKine®

Mfr.No.

EK1598

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.107	0.165	0.201	0.296	0.475	0.736	1.236	1.790

Description

Human Bikunin / alpha 1 Microglobulin ELISA Kit PicoKine® (96 Tests). Quantitate Human AMBP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Protein AMBP; Alpha-1-microglobulin; Protein HC; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge; Inter-alpha-trypsin inhibitor light chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-acid-rich protein; Trypstatin; AMBP; HCP; ITIL

Immunogen

Expression system for standard: NS0; Immunogen sequence: A206-N352

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Bikunin

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	38	243	418	36	264	406
Standard deviation	2.35	16.03	25.08	2.48	18.48	26.2%7
CV (%)	6.2%	6.6%	6%	6.9%	7%	6.2%

Protein Function

Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Background

Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	38	40	35	40	38	2.04	5.3%
Sample 2	243	252	272	236	250	13.51	5.4%
Sample 3	418	447	460	414	434	19.35	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	259
Antigen	Bikunin / alpha 1 Microglobulin
Gene Name	AMBP
UniProt ID	P02760
Tissue Specificity	Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha- trypsin inhibitor is present in plasma and urine.

Applications	ELISA
Research Area	Cancer,Cardiovascular,Cell Biology,Protease Inhibitors,Proteolysis/Ubiquitin,Serum Proteins,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001306	EK1598	96 wells/kit	$499		Add to Cart

Human Bikunin / alpha 1 Microglobulin ELISA Kit PicoKine®

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