Human beta IG-H3/TGFBI ELISA Kit PicoKine®

Mfr.No.

EK1202

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.044	0.068	0.107	0.173	0.353	0.674	1.198	2.028

Description

Human beta IG-H3/TGFBI ELISA Kit PicoKine® (96 Tests). Quantitate Human TGFBI in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transforming growth factor-beta-induced protein ig-h3;Beta ig-h3;Kerato-epithelin;RGD-containing collagen-associated protein;RGD-CAP;TGFBI;BIGH3;

Molecular Weight

74681 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G24-H683

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TGFBI

Subcellular Localization

Secreted, extracellular space, extracellular matrix. May be associated both with microfibrils and with the cell surface.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	154	388	1132	160	387	1210
Standard deviation	6.77	28.32	57.73	7.36	34.41	70.18
CV (%)	4.4%	7.3%	5.8%	4.6%	9.1%	5.8%

Protein Function

Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.

Background

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	154	145	147	157	150	4.91	3.2%
Sample 2	388	396	437	439	415	23.18	5.5%
Sample 3	1132	1229	1284	1106	1187	72.03	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7045
Antigen	IG-H3/TGFBI
Gene Name	TGFBI
UniProt ID	Q15582
Protein Name	Transforming growth factor-beta-induced protein ig-h3
Tissue Specificity	Highly expressed in the corneal epithelium. .
Sequence	Contains 1 EMI domain.

Applications	ELISA
Research Area	Collagen,Cytoskeleton/ECM,Developmental Biology,ECM Proteins,Extracellular Matrix,Growth Factors/Hormones,Neuroscience,Organogenesis,Sensory System,Signal Transduction,Structures,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000819	EK1202	96 wells/kit	$499		Add to Cart

Human beta IG-H3/TGFBI ELISA Kit PicoKine®

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