Human B7-H2/ICOSL ELISA Kit PicoKine®

Mfr.No.

EK2217

Sensitivity

<300 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.019	0.115	0.193	0.410	0.737	1.176	1.355	2.053

Description

Human B7-H2/ICOSL PicoKine® ELISA Kit (96 Tests). Quantitate Human B7-H2/ICOSL in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 300 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-S258

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ICOSLG

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	2015	4613	23467	1987	4563	21547
Standard deviation	90.68	281.39	1608	97.36	333.1	1335.91
CV(%)	4.5%	6.1%	6.8%	4.9%	7.3%	6.2%

Background

ICOS ligand (ICOSLG, ICOSL or GL50) is a protein that in humans is encoded by the ICOSLG gene located at chromosome 21. Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2015	2162	1984	1947	2027	99.32	4.9%
Sample 2	4613	4696	4536	4799	4661	270.34	5.8%
Sample 3	23647	25479	24519	22796	24020	1393.17	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	23308
Antigen	B7-H2/ICOSL
Gene Name	ICOSLG
UniProt ID	O75144

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001764	EK2217	96 wells/kit	$499		Add to Cart

Human B7-H2/ICOSL ELISA Kit PicoKine®

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