Matrices listed below were spiked with certain level of ATXN1 and the recovery rates were calculated by comparing the measured value to the expected amount of ATXN1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
96
EDTA plasma(n=5)
86-95
90
heparin plasma(n=5)
86-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATXN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ATXN1 ELISA Kit allows for the in vitro quantitative determination of ATXN1 concentrations in serum, plasma, tissue homogenates and other biological fluids.