Human Atrophin-1 - ATN1 ELISA Kit

Mfr.No.

E4137hu

Sensitivity

4.94ng/L

Detection Range

10-3500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Atrophin-1 (also known as ATN1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ATN 1;ATN1;Atrophin-1;Dentatorubral-pallidoluysian atrophy protein

Molecular Weight

125414

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4137hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	428.7	30.92	7.2
Intra-Assay	2	18	1133.9	64.31	5.7
Intra-Assay	3	18	196.8	15.52	7.9

Background

Transcriptional corepressor. Recruits NR2E1 to repress transcription. Promotes vascular smooth cell (VSMC) migration and orientation (By similarity). Corepressor of MTG8 transcriptional repression. Has some intrinsic repression activity which is independent of the number of poly-Gln (polyQ) repeats.

Protocol

These standard curves of E4137hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.26	2.2	2.167
	2.14
1000ng/L	1.719	1.619	1.586
	1.518
500ng/L	1.014	1.02	0.987
	1.027
250ng/L	0.69	0.666	0.633
	0.641
125ng/L	0.414	0.418	0.385
	0.422
0ng/L	0.036	0.033	0
	0.03

Gene ID	1822
UniProt ID	P54259

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105539	E4137hu	48T	$320		Add to Cart
BHE12105539	E4137hu	96T	$458		Add to Cart

Human Atrophin-1 - ATN1 ELISA Kit

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