Human Atrial Natriuretic Peptide-converting Enzyme

Mfr.No.

E1043Hu

Description

This sandwich kit is for the accurate quantitative detection of human Atrial Natriuretic Peptide-converting Enzyme (also known as CORIN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ATC2;Atrial natriuretic Peptide-converting enzyme;Atrial natriuretic Peptide-converting enzyme, 100 kDa soluble fragment);Atrial natriuretic Peptide-converting enzyme, 160 kDa soluble fragment;Atrial natriuretic Peptide-converting enzyme, 180 kDa soluble fragment;Atrial natriuretic Peptide-converting enzyme, activated protease fragment;Atrial natriuretic Peptide-converting enzyme, N-terminal propeptide;CORIN;CRN;Heart-specific serine proteinase ATC2;Lrp4;PEE5;Pro-ANP-converting enzyme;TMPRSS10;Transmembrane protease serine 10

Reactivity

Human

Detection Range

0.1-40ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

116486

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.05ng/ml

Background

Serine-type endopeptidase involved in atrial natriuretic peptide (NPPA) and brain natriuretic peptide (NPPB) processing (PubMed:10880574, PubMed:21288900, PubMed:20489134, PubMed:21763278). Converts through proteolytic cleavage the non-functional propeptides NPPA and NPPB into their active hormones, ANP and BNP(1-32) respectively, thereby regulating blood pressure in the heart and promoting natriuresis, diuresis and vasodilation (PubMed:10880574, PubMed:21288900, PubMed:20489134, PubMed:21763278). Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus (PubMed:22437503). Also acts as a regulator of sodium reabsorption in kidney (By similarity). has weaker endopeptidase activity compared to isoform 1.

Gene ID	10699
UniProt ID	Q9Y5Q5
Antigen	CORIN

Research Area

Cell Biology

Protocol

These standard curves of E1043Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.276	2.281	2.259
	2.285
12ng/ml	1.257	1.241	1.22
	1.225
6ng/ml	0.712	0.717	0.695
	0.721
3ng/ml	0.377	0.409	0.388
	0.442
1.5ng/ml	0.13	0.153	0.132
	0.176
0ng/ml	0.018	0.021	0
	0.025

Precision

We measured random samples of E1043Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.83	0.32	4.09
Intra-Assay	2	18	12.57	0.32	2.57
Intra-Assay	3	18	3.47	0.2	5.76

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12102700	E1043Hu	48T	$320		Add to Cart
BHE12102700	E1043Hu	96T	$458		Add to Cart

Human Atrial Natriuretic Peptide-converting Enzyme - CORIN ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Atrial Natriuretic Peptide-converting Enzyme - CORIN ELISA Kit

Rate it now..!!