Human Astacin Like Metallo Endopeptidase

Mfr.No.

E2833Hu

Sensitivity

0.022ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Astacin-like metalloendopeptidase;ASTL;Oocyte astacin;Ovastacin

Molecular Weight

45936

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2833Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3	0.2	6.7
Intra-Assay	2	18	5.5	0.25	4.6
Intra-Assay	3	18	1	0.07	6.8

Background

Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity).

Protocol

These standard curves of E2833Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.273	2.218	2.174
	2.163
6ng/ml	1.569	1.654	1.61
	1.739
3ng/ml	1.104	1.046	1.002
	0.988
1.5ng/ml	0.69	0.722	0.677
	0.753
0.75ng/ml	0.402	0.401	0.356
	0.4
0ng/ml	0.034	0.044	0
	0.055

Gene ID	431705
UniProt ID	Q6HA08

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104370	E2833Hu	48T	$320		Add to Cart
BHE12104370	E2833Hu	96T	$458		Add to Cart

Human Astacin Like Metallo Endopeptidase - ASTL ELISA Kit

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