Human Aspartyl/Asparaginyl Beta-hydroxylase

Mfr.No.

E4537Hu

Alternative Names

ASP beta-hydroxylase;Aspartate beta-hydroxylase;Aspartyl/asparaginyl beta-hydroxylase;ASPH;Peptide-aspartate beta-dioxygenase

Reactivity

Human

Detection Range

2-600ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

85863

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.24ng/L

Background

(Isoform 1): specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. (Isoform 8): membrane-bound Ca(2+)-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) Channels in T-cells.

Gene ID	444
UniProt ID	Q12797
Antigen	ASPH

Research Area

Cell Biology

Protocol

These standard curves of E4537Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.118	2.138	2.093
	2.158
160ng/L	1.447	1.409	1.364
	1.371
80ng/L	0.607	0.612	0.567
	0.618
40ng/L	0.315	0.374	0.329
	0.434
20ng/L	0.137	0.173	0.128
	0.208
0ng/L	0.045	0.045	0
	0.044

Precision

We measured random samples of E4537Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	152.7	4.49	2.9
Intra-Assay	2	18	121.8	5.44	4.5
Intra-Assay	3	18	56.6	2.06	3.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105853	E4537Hu	48T	$320		Add to Cart
BHE12105853	E4537Hu	96T	$458		Add to Cart

Human Aspartyl/Asparaginyl Beta-hydroxylase - ASPH ELISA Kit

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