Human Aryl Hydrocarbon Receptor Repressor

Mfr.No.

E2832Hu

Description

This sandwich kit is for the accurate quantitative detection of human Aryl Hydrocarbon Receptor Repressor (also known as AHRR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AhR repressor;AHRR;Aryl hydrocarbon receptor repressor;bHLHe77;Class E basic helix-loop-helix protein 77

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

76265

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.35ng/L

Background

Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site.

Gene ID	57491
UniProt ID	A9YTQ3
Antigen	AHRR

Research Area

Others

Protocol

These standard curves of E2832Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.27	2.277	2.224
	2.284
600ng/L	1.304	1.39	1.337
	1.475
300ng/L	0.672	0.719	0.666
	0.766
150ng/L	0.512	0.505	0.452
	0.499
75ng/L	0.285	0.258	0.205
	0.232
0ng/L	0.046	0.053	0
	0.06

Precision

We measured random samples of E2832Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	756.7	20.05	2.6
Intra-Assay	2	18	133.2	8.46	6.4
Intra-Assay	3	18	536.4	28.64	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104369	E2832Hu	48T	$320		Add to Cart
BHE12104369	E2832Hu	96T	$458		Add to Cart

Human Aryl Hydrocarbon Receptor Repressor - AHRR ELISA Kit

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