Human Arf-Gap with Sh3 Domain - Ank Repeat and Ph Domain-Coutaining Protein 1

Mfr.No.

E4128hu

Description

This sandwich kit is for the accurate quantitative detection of human Arf-GAP With SH3 Domain,ANK Repeat and PH Domain-Coutaining Protein I (also known as ASAP I) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein;ADP-ribosylation factor-directed GTPase-activating protein 1;AMAP1;ARF GTPase-activating protein 1;Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1;ASAP1;CENTB4;DDEF1;DEF-1;Development and differentiation-enhancing factor 1;Differentiation-enhancing factor 1;PAG2;PAP;PIP2-dependent ARF1 GAP;ZG14P

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

125498

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.91ng/L

Background

Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.

Gene ID	50807
UniProt ID	Q9ULH1
Antigen	ASAP1

Research Area

Tags & Cell Markers

Protocol

These standard curves of E4128hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.179	2.189	2.133
	2.199
1200ng/L	1.177	1.213	1.157
	1.249
600ng/L	0.805	0.717	0.661
	0.63
300ng/L	0.437	0.429	0.373
	0.421
150ng/L	0.175	0.176	0.12
	0.177
0ng/L	0.063	0.056	0
	0.049

Precision

We measured random samples of E4128hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	503.37	35.35	7.02
Intra-Assay	2	18	1085.03	60.84	5.61
Intra-Assay	3	18	1662.64	48.75	2.93

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105532	E4128hu	48T	$320		Add to Cart
BHE12105532	E4128hu	96T	$458		Add to Cart

Human Arf-Gap with Sh3 Domain - Ank Repeat and Ph Domain-Coutaining Protein 1 - ASAP1 ELISA Kit

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