Human Arachidonate 5-lipoxygenase-activating Protein

Mfr.No.

E4982Hu

Sensitivity

1.41ng/L

Detection Range

3-900ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ALOX5AP;Arachidonate 5-lipoxygenase-activating protein;FLAP;MK-886-binding protein

Molecular Weight

18157

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4982Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	124.6	7.69	6.2
Intra-Assay	2	18	241.9	12.14	5
Intra-Assay	3	18	62.6	3.99	6.4

Background

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.

Protocol

These standard curves of E4982Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.374	2.319	2.263
	2.265
240ng/L	1.722	1.753	1.697
	1.785
120ng/L	1.205	1.171	1.114
	1.136
60ng/L	0.69	0.653	0.596
	0.615
30ng/L	0.381	0.406	0.349
	0.43
0ng/L	0.054	0.057	0
	0.06

Gene ID	241
UniProt ID	P20292

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106255	E4982Hu	48T	$320		Add to Cart
BHE12106255	E4982Hu	96T	$458		Add to Cart

Human Arachidonate 5-lipoxygenase-activating Protein - ALOX5AP ELISA Kit

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