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Home / ELISA Kit

Human Aquaporin 3 (AQP3) ELISA Kit

SKU : BHE13704417

AQP3 encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of AQP3 results in multiple transcript variants encoding different isoforms.

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Mfr.No.	KTE62203
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Aquaporin 3 (AQP3) ELISA Kit employs a two-site sandwich ELISA to quantitate AQP3.
Alternative Names	AQP3; GIL; Gill blood group; aquaporin 3; aquaporin 3 (GIL blood group)
Detection Method	Colorimetric
Kit Components	Human Aquaporin 3 microplate Human Aquaporin 3 standard Human Aquaporin 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Aquaporin 3 (AQP3) ELISA Kit has high sensitivity and excellent specificity for detection of Human AQP3. No significant cross-reactivity or interference between Human AQP3 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	AQP3 encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alteative splicing of AQP3 results in multiple transcript variants encoding different isoforms.
Notes	This Human Aquaporin 3 (AQP3) ELISA Kit employs a two-site sandwich ELISA to quantitate AQP3 in samples. An antibody specific for AQP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAQP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AQP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	360

Applications	ELISA
Applications Notes	This Human Aquaporin 3 (AQP3) ELISA Kit employs a two-site sandwich ELISA to quantitate AQP3 in samples. An antibody specific for AQP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAQP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AQP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704417	KTE62203	48 T	$359		Add to Cart
BHE13704417	KTE62203	96 T	$619		Add to Cart
BHE13704417	KTE62203	96 Tx5	$2809		Add to Cart
BHE13704417	KTE62203	96 Tx50	$26209		Add to Cart

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