Human APOE/Apolipoprotein E ELISA Kit PicoKine®

Mfr.No.

EK1455

Sensitivity

<50 pg/ml

Detection Range

3.12 ng/ml - 200 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.010	0.073	0.138	0.243	0.484	0.907	1.475	2.004

Description

Human APOE/Apolipoprotein E ELISA Kit PicoKine® (96 Tests). Quantitate Human APOE in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva, milk and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Apolipoprotein E;Apo-E;APOE;

Molecular Weight

36154 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: K19-H317

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural human APOE

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Milk, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	7334	30860	101114	7480	33927	97046
Standard deviation	506.04	2191.06	7886.89	598.4	2917.72	7957.77
CV (%)	6.9%	7.1%	7.8%	8%	8.6%	8.2%

Protein Function

Mediates the binding, intealization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

Background

Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimers disease and cardiovascular disease.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	7334	7205	6656	6991	7046	256.58	3.6%
Sample 2	30860	28598	30251	27221	29232	1426.08	4.8%
Sample 3	101114	104676	102789	88986	99391	6138.2	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	348
Gene Name	APOE
UniProt ID	P02649
Protein Name	Apolipoprotein E
Tissue Specificity	Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Applications	ELISA
Research Area	Cardiovascular,Lipid Metabolism,Lipids/Lipoproteins,Metabolism,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001061	EK1455	96 wells/kit	$499		Add to Cart

Human APOE/Apolipoprotein E ELISA Kit PicoKine®

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