Human APOA1/Apolipoprotein A-I ELISA Kit PicoKine®

Mfr.No.

EK1456

Sensitivity

<50 pg/ml

Detection Range

3.12 ng/ml - 200 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.067	0.121	0.170	0.283	0.522	1.047	1.738	1.899

Description

Human APOA1 / Apoa I / Apolipoprotein AI ELISA Kit PicoKine® (96 Tests). Quantitate Human APOA1 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Apolipoprotein A-I;Apo-AI;ApoA-I;Apolipoprotein A1;Proapolipoprotein A-I;ProapoA-I;Truncated apolipoprotein A-I;Apolipoprotein A-I (1-242);APOA1;

Molecular Weight

30778 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D25-Q267

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural human APOA1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	8186	19826	93780	8183	19180	85400
Standard deviation	376.55	1447.29	4876.56	507.34	1514.62	5209.4
CV (%)	4.6%	7.3%	5.2%	6.2%	7.9%	6.1%

Protein Function

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. .

Background

Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alteative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	8186	7996	8002	7330	7878	325.75	4.1%
Sample 2	19826	19748	20656	19657	19971	399.55	2%
Sample 3	93780	86259	87325	86965	88582	3025.32	3.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	335
Gene Name	APOA1
UniProt ID	P02647
Protein Name	Apolipoprotein A-I
Tissue Specificity	Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. .

Applications	ELISA
Research Area	Cardiovascular,Cholesterol Metabolism,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001062	EK1456	96 wells/kit	$499		Add to Cart

Human APOA1/Apolipoprotein A-I ELISA Kit PicoKine®

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