Human AP13(Apelin 13) ELISA Kit

Mfr.No.

ELK2252

Sensitivity

8.24 pg/mL

Detection Range

25-1600 pg/mL

Reactivity

Human

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

APLN13

Recovery

Matrices listed below were spiked with certain level of recombinant AP13 and the recovery rates were calculated by comparing the measured value to the expected amount of AP13 in samples.

Matrix	Recovery range	Average
serum(n=5)	77-95%	86%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	88-103%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AP13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	87-96%	95-103%	85-91%
EDTA plasma(n=5)	85-92%	90-96%	95-102%	87-98%
Heparin plasma(n=5)	93-102%	90-99%	95-106%	87-101%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1600.00	0.227
800.00	0.386
400.00	0.583
200.00	0.857
100.00	1.152
50.00	1.511
25.00	1.789
0.00	2.115

Limit Of Detection

1600 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200726	ELK2252	48T	$320		Add to Cart
BHE15200726	ELK2252	96T	$458		Add to Cart

Human AP13(Apelin 13) ELISA Kit

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