Human anti-insulin receptor antibody,AIRA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09568h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Sample Volume

50-100ul

Alternative Names

anti-insulin receptor antibody,AIRA

Recovery

The recovery of rat GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	92	87-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	85
	Range %	80-90
1:400	Average %	102
	Range %	97-107
1:800	Average %	87
	Range %	83-91
1:1600	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	InsR Ab

Storage	Store at 2-8°C. Please refer to protocol.

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