Human Anoctamin 1 - ANO1 ELISA Kit

Mfr.No.

E2371Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Anoctamin 1 (also known as ANO1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ANO 1;ANO1;Anoctamin-1;Discovered on gastrointestinal stromal tumors protein 1;Oral cancer overexpressed protein 2;Transmembrane protein 16A;Tumor-amplified and overexpressed sequence 2

Reactivity

Human

Detection Range

0.05-30ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

114078

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.022ng/ml

Background

Calcium-activated chloride Channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.

Gene ID	55107
UniProt ID	Q5XXA6
Antigen	ANO1

Research Area

Others

Protocol

These standard curves of E2371Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.107	2.148	2.114
	2.189
8ng/ml	1.35	1.438	1.404
	1.525
4ng/ml	0.552	0.619	0.585
	0.687
2ng/ml	0.457	0.403	0.369
	0.348
1ng/ml	0.162	0.179	0.145
	0.195
0ng/ml	0.029	0.034	0
	0.039

Precision

We measured random samples of E2371Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.9	0.3	3.4
Intra-Assay	2	18	3.4	0.17	4.8
Intra-Assay	3	18	4.7	0.23	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103909	E2371Hu	48T	$320		Add to Cart
BHE12103909	E2371Hu	96T	$458		Add to Cart

Human Anoctamin 1 - ANO1 ELISA Kit

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