Human Ankyrin Repeat and Socs Box Protein 2

Mfr.No.

E4952Hu

Alternative Names

Ankyrin repeat and SOCS box protein 2;ASB 2;ASB2;ASB-2

Reactivity

Human

Detection Range

5-1000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

65084

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.04ng/L

Background

Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. (Isoform 1): Enhances adhesion of hematopoietic cells to fibronectin through targeting of filamins FLNA and FLNB for proteasomal degradation, it is transiantly expressed during hematopoietic cell differentiation. (Isoform 2): Involved in myogenic differentiation and targets filamin FLNB for proteasomal degradation but not filamin FLNA.

Gene ID	51676
UniProt ID	Q96Q27
Antigen	ASB2

Research Area

Others

Protocol

These standard curves of E4952Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.469	2.49	2.437
	2.511
320ng/L	1.388	1.412	1.359
	1.437
160ng/L	0.755	0.784	0.731
	0.814
80ng/L	0.464	0.448	0.395
	0.433
40ng/L	0.234	0.256	0.204
	0.279
0ng/L	0.043	0.053	0
	0.063

Precision

We measured random samples of E4952Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	384.3	9.23	2.4
Intra-Assay	2	18	242.9	10.57	4.4
Intra-Assay	3	18	106.8	5.27	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106225	E4952Hu	48T	$320		Add to Cart
BHE12106225	E4952Hu	96T	$458		Add to Cart

Human Ankyrin Repeat and Socs Box Protein 2 - ASB2 ELISA Kit

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