Human ANGPTL7 ELISA Kit PicoKine®

Mfr.No.

EK2035

Sensitivity

<50 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.044	0.109	0.162	0.268	0.480	0.828	1.459	2.048

Description

Human ANGPTL7 ELISA Kit PicoKine® (96 Tests). Quantitate Human ANGPTL7 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Angiopoietin-related protein 7; Angiopoietin-like factor; Angiopoietin-like protein 7; Coea-derived transcript 6 protein; ANGPTL7; CDT6; UNQ313; PRO356

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q27-P346

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ANGPTL7

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	140	644	1907	145	639	2253
Standard deviation	6.86	27.69	97.26	9.57	35.15	173.48
CV (%)	4.9%	4.3%	5.1%	6.6%	5.5%	7.7%

Background

Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 1p36.22. Angiopoietin-like Protein 7 (ANGPTL7), also known as Coeal-Derived Transcript 6 (CDT6), is a secreted, 45?50 kDa glycoprotein member of the angiopoietin?like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the coeal stroma, trabecular meshwork, and sclera. Its production is up?regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. ANGPTL7 expression is up?regulated under conditions of increased ocular pressure.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	140	129	118	129	129	7.77	6.0%
Sample 2	644	660	702	583	647	42.71	6.6%
Sample 3	1907	2152	2078	2215	2088	115.20	5.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10218
Gene Name	ANGPTL7
UniProt ID	O43827
Protein Name	Angiopoietin-related protein 7
Tissue Specificity	Highly and specifically expressed in the coea where is confined to the stromal layer.

Applications	ELISA
Research Area	Neuroscience,Secreted,Sensory System,Signaling Pathways,Stem Cells,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001532	EK2035	96 wells/kit	$499		Add to Cart

Human ANGPTL7 ELISA Kit PicoKine®

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