Human ANGPTL3 ELISA Kit PicoKine®

Mfr.No.

EK1374

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.012	0.158	0.251	0.447	0.807	1.266	1.575	2.066

Description

Human ANGPTL3 ELISA Kit PicoKine® (96 Tests). Quantitate Human ANGPTL3 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Angiopoietin-related protein 3;Angiopoietin-5;ANG-5;Angiopoietin-like protein 3;ANGPTL3;ANGPT5;UNQ153/PRO179;

Molecular Weight

53637 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S17-E460

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ANGPTL3

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	704	1869	9766	766	1705	9664
Standard deviation	32.38	110.27	556.66	46.72	121.05	570.17
CV (%)	4.6%	5.7%	5.7%	6.1%	7.1%	5.9%

Background

ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	704	723	638	687	688	31.55	4.5%
Sample 2	1869	2168	2187	1992	2054	131.1	6.3%
Sample 3	9766	8411	10192	8397	9191	801.78	8.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	27329
Gene Name	ANGPTL3
UniProt ID	Q9Y5C1
Protein Name	Angiopoietin-related protein 3
Tissue Specificity	Expressed principally in liver. Weakly expressed in kidney. .
Sequence	Contains 1 fibrinogen C-terminal domain.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Energy Metabolism,Energy Transfer Pathways,Growth Factors,Lipid Metabolism,Metabolic Signaling Pathways,Metabolism,Obesity,Pathways and Processes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000963	EK1374	96 wells/kit	$499		Add to Cart

Human ANGPTL3 ELISA Kit PicoKine®

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