Human Angiopoietin-like Protein 8

Mfr.No.

E3372Hu

Sensitivity

2.36ng/L

Detection Range

5-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Angiopoietin-like Protein 8 (also known as ANGPTL8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Angiopoietin-like protein 8;ANGPTL 8;ANGPTL8;Betatrophin;Lipasin;Refeeding-induced fat and liver protein

Molecular Weight

22105

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3372Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	856.3	25.93	3
Intra-Assay	2	18	253	15.43	6.1
Intra-Assay	3	18	490	25.41	5.2

Background

Hormone that acts as a blood lipid regulator by regulating serum triglyceride levels (PubMed: 22569073, PubMed: 22809513, PubMed: 23150577). May be involved in the metabolic transition between fasting and refeeding: required to direct fatty acids to adipose tissue for storage in the fed state (By similarity).

Protocol

These standard curves of E3372Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.231	2.138	2.1
	2.046
600ng/L	1.344	1.301	1.263
	1.258
300ng/L	0.76	0.714	0.676
	0.668
150ng/L	0.525	0.527	0.489
	0.529
75ng/L	0.199	0.191	0.153
	0.183
0ng/L	0.036	0.038	0
	0.041

Gene ID	55908
UniProt ID	Q6UXH0

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104901	E3372Hu	48T	$320		Add to Cart
BHE12104901	E3372Hu	96T	$458		Add to Cart

Human Angiopoietin-like Protein 8 - ANGPTL8 ELISA Kit

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