Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine®

Mfr.No.

EK0657

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.095	0.178	0.210	0.376	0.519	0.970	1.588	2.010

Description

Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine® (96 Tests). Quantitate Human ANGPT2 in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Angiopoietin-2;ANG-2;ANGPT2;

Molecular Weight

56919 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y19-F496

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Angiopoietin-2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	327	1634	5900	341	1769	5438
Standard deviation	17.33	114.38	354	23.52	127.36	342.59
CV (%)	5.3%	7%	6%	6.9%	7.2%	6.3%

Protein Function

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. .

Background

Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	327	351	360	317	338	17.41	5.1%
Sample 2	1634	1902	1611	1928	1768	146.76	8.3%
Sample 3	5900	6201	5314	5672	5771	324.1	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	285
Gene Name	ANGPT2
UniProt ID	O15123
Protein Name	Angiopoietin-2
Sequence	Contains 1 fibrinogen C-terminal domain.

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Growth Factors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000547	EK0657	96 wells/kit	$499		Add to Cart

Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine®

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