Human Angiopoietin 2 - ANG-2 ELISA Kit

Mfr.No.

E1221Hu

Description

This sandwich kit is for the accurate quantitative detection of human Angiopoietin 2 (also known as ANG-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ANG 2;ANG-2;Angiopoietin-2

Reactivity

Human

Detection Range

5-1000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

56919

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.49ng/L

Background

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Gene ID	285
UniProt ID	O15123
Antigen	ANG-2

Research Area

Cardiovascular

Protocol

These standard curves of E1221Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	1.945	1.825	1.77
	1.706
320ng/L	1.061	1.041	0.986
	1.021
160ng/L	0.535	0.567	0.512
	0.599
80ng/L	0.289	0.237	0.182
	0.185
40ng/L	0.135	0.12	0.065
	0.106
0ng/L	0.059	0.056	0
	0.052

Precision

We measured random samples of E1221Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	528.7	16.52	3.1
Intra-Assay	2	18	201.8	9.55	4.7
Intra-Assay	3	18	354.8	18.19	5.1

Storage	2-8°C

Reference	Effect of Vitamin E on Serum Levels of Vascular Endothelial Growth Factor and Angiopoietin-1 in Women with Polycystic Ovary Syndrome: A Pilot Randomized, Placebo-Controlled Trial. Journal: International Journal of Fertility & Sterility

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102867	E1221Hu	48T	$320		Add to Cart
BHE12102867	E1221Hu	96T	$458		Add to Cart

Human Angiopoietin 2 - ANG-2 ELISA Kit

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