Human Angiogenin/ANG ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0305

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	78	156	313	625	1250	2500	5000
O.D.	0.047	0.116	0.169	0.265	0.506	0.927	1.534	2.375

Description

Human Angiogenin/ANG ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ANG in cell culture supeatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Alternative Names

Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5;

Molecular Weight

16550 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q25-P147

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Angiogenin/ANG

Subcellular Localization

Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.

Sample Type

Cell culture supeatants, Cell lysates, Tissue homogenates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	312	672	1663	635	1856	3489
Standard deviation	9.4	23.52	54.9	45.72	165.2	300
CV (%)	3%	3.5%	3.3%	7.2%	8.9%	8.6%

Protein Function

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .

Background

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	283
Gene Name	ANG
UniProt ID	P03950
Protein Name	Angiogenin
Tissue Specificity	Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .
Sequence	Belongs to the pancreatic ribonuclease family.

Applications	ELISA
Research Area	Actin Binding Proteins,Actin, etc.,Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Cytoskeleton,Cytoskeleton/ECM,DNA/RNA,Epigenetics and Nuclear Signaling,Hypoxia,Invasion/Microenvironment,Metabolism,Metabolism Processes,Microfilaments,Neurodegenerative Disease,Neurology Process,Neuroscience,Pathways and Processes,Response To Hypoxia,RNA Processing,Signal Transduction,Translation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000002	EZ0305	5 plates/kit	$500		Add to Cart

Human Angiogenin/ANG ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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