Matrices listed below were spiked with certain level of AMPK and the recovery rates were calculated by comparing the measured value to the expected amount of AMPK in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
94
EDTA plasma(n=5)
89-100
94
heparin plasma(n=5)
92-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMPK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AMPK ELISA Kit allows for the in vitro quantitative determination of AMPK concentrations in serum, plasma, tissue homogenates and other biological fluids.