Human AMICA/JAML ELISA Kit PicoKine®

Mfr.No.

EK1730

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.015	0.086	0.123	0.209	0.430	0.720	1.217	1.890

Description

Human AMICA/JAML ELISA Kit PicoKine® (96 Tests). Quantitate Human JAML in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Junctional adhesion molecule-like; Adhesion molecule interacting with CXADR antigen 1; Dendritic cell-specific protein CREA7-1; JAML; AMICA1; UNQ722; PRO1387

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-L275

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human AMICA/JAML

Subcellular Localization

Cell membrane, Single-pass type I membrane protein, Cell junction

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	106	451	1179	110	500	1230
Standard deviation	4.45	20.8	62.5	7.04	34	88.56
CV (%)	4.2%	4.6%	5.3%	6.4%	6.8%	7.2%

Protein Function

Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors me

Background

JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member. This gene is mapped to 11q23.3. It is composed of two extracellular immunoglobulin-like domains, a membrane-spanning region, and a cytoplasmic tail involved in activation signaling. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in humans and CAR in mice) which has been shown to localize to the tight junctions of epithelial cells. JAML-mediated activation of CAR is required for neutrophil extravasation in addition to other leukocyte/epithelial cell interaction models. Other members of the JAM family of transmembrane proteins include JAM1, JAM2 and JAM3.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	106	110	110	107	108	1.78	1.6%
Sample 2	451	458	536	485	482	33.39	6.9%
Sample 3	1179	1111	1036	1140	1116	52.36	4.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	120425
Gene Name	JAML
UniProt ID	Q86YT9
Tissue Specificity	Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. Preferentially expressed by mature leukocytes.

Applications	ELISA
Research Area	Cell Adhesion,Cell Adhesion Molecules,Cell Type Markers,Cytoskeleton/ECM,Immunology,Signal Transduction,Tight Junctions

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001388	EK1730	96 wells/kit	$499		Add to Cart

Human AMICA/JAML ELISA Kit PicoKine®

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